New Conjugated Polymers for Photoinduced Unwinding of DNA Supercoiling and Gene Regulation
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- 作者：Gaomai Yang ; Huanxiang Yuan ; Chunlei Zhu ; Libing Liu ; Qiong Yang ; Fengting Lv ; Shu Wang
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2012
- 出版时间：May 23, 2012
- 年：2012
- 卷：4
- 期：5
- 页码：2334-2337
- 全文大小：272K
- 年卷期：v.4,no.5(May 23, 2012)
- ISSN：1944-8252
文摘
Three cationic polythiophene derivatives (P1, P2, P3) were synthesized and characterized. Under white light irradiation (400鈥?00 nm), they sensitize oxygen molecule in the surrounding to generate reactive oxygen species (ROS) that can efficiently unwind the supercoiled DNA in vitro. Further study shows that this relaxation of the DNA supercoiling results in the decrease of gene (pCX-EGFP plasmid) expression level. The ability of these conjugated polymers for regulating gene expression will add a new dimension to the function of conjugated polymers.