Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles
详细信息 查看全文
- 作者：César Barrios-Collado ; Diego García-Gómez ; Renato Zenobi ; Guillermo Vidal-de-Miguel ; Alfredo J. Ibáñez ; Pablo Martinez-Lozano Sinues
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：February 16, 2016
- 年：2016
- 卷：88
- 期：4
- 页码：2406-2412
- 全文大小：461K
- ISSN：1520-6882
文摘
We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day–night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics. To validate the soundness of our in vivo measurements, we fully characterized some key compounds via tandem mass spectrometry (MS/MS) and confirmed their expected behavior based on prior gas chromatography/mass spectrometry (GC/MS) studies. For example, β-caryophyllene, which is directly related to photosynthesis, was found to show a periodic day–night pattern with highest concentrations during the day. We conclude that the capability of SESI-MS to capture highly dynamic VOC emissions and wide analyte coverage makes it an attractive tool to complement GC/MS in plant studies.