Versatile Fluorescence Resonance Energy Transfer-Based Mesoporous Silica Nanoparticles for Real-Time Monitoring of Drug Release
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- 作者：Jinping Lai ; Birju P. Shah ; Eric Garfunkel ; Ki-Bum Lee
- 刊名：ACS Nano
- 出版年：2013
- 出版时间：March 26, 2013
- 年：2013
- 卷：7
- 期：3
- 页码：2741-2750
- 全文大小：640K
- 年卷期：v.7,no.3(March 26, 2013)
- ISSN：1936-086X
文摘
We describe the development of a versatile fluorescence resonance energy transfer (FRET)-based real-time monitoring system, consisting of (a) coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) as the drug carrier, (b) a fluorescein isothiocyanate-尾-cyclodextrin (FITC-尾-CD) as redox-responsive molecular valve blocking the pores, and (c) a FRET donor鈥揳cceptor pair of coumarin and FITC integrated within the pore-unlocking event, thereby allowing for monitoring the release of drugs from the pores in real-time. Under nonreducing conditions, when the disulfide bond is intact, the close proximity between coumarin and FITC on the surface of MSNs results in FRET from coumarin to FITC. However, in the presence of the redox stimuli like glutathione (GSH), the disulfide bond is cleaved which leads to the removal of molecular valve (FITC-尾-CD), thus triggering drug release and eliminating FRET. By engineering such a FRET-active donor鈥揳cceptor structure within the redox-responsive molecular valve, we can monitor the release of the drugs entrapped within the pores of the MSN nanocarrier, following the change in the FRET signal. We have demonstrated that, any exogenous or endogenous change in the GSH concentration will result in a change in the extent of drug release as well as a concurrent change in the FRET signal, allowing us to extend the applications of our FRET-based MSNs for monitoring the release of any type of drug molecule in real-time.