Rapid and Semiautomated Method for the Analysis of Veterinary Drug Residues in Honey Based on Turbulent-Flow Liquid Chromatography Coupled to Ultrahigh-Performance Liquid Chromatography鈥揙rbitrap Mass
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- 作者：Mar铆a M. Aguilera-Luiz ; Roberto Romero-Gonz谩lez ; Patricia Plaza-Bola帽os ; Jos茅 L. Mart铆nez Vidal ; Antonia Garrido Frenich
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：January 30, 2013
- 年：2013
- 卷：61
- 期：4
- 页码：829-839
- 全文大小：530K
- 年卷期：v.61,no.4(January 30, 2013)
- ISSN：1520-5118
文摘
A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography鈥揙rbitrap mass spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 渭g/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <25% for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 渭g/kg and limits of identification (LOIs) from 0.1 to 50 渭g/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.