Determination of Ascorbic Acid and Carotenoids in Food Commodities by Liquid Chromatography with Mass Spectrometry Detection
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- 作者：A. Garrido Frenich ; M. E. Herná ; ndez Torres ; A. Belmonte Vega ; J. L. Martí ; nez Vidal ; and P. Plaza Bolañ ; os
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：September 21, 2005
- 年：2005
- 卷：53
- 期：19
- 页码：7371 - 7376
- 全文大小：91K
- 年卷期：v.53,no.19(September 21, 2005)
- ISSN：1520-5118
文摘
Two methods, one to determine ascorbic acid and one to determine lycopene and 
-carotene, invegetables and fruits by liquid chromatography coupled with mass spectrometry (LC-MS) have beenestablished. The chromatographic separation of the studied compounds and their MS parameterswere optimized to improve selectivity and sensitivity. In both methods, separation was carried outwith two coupled columns, first a C18 and then a dC18, using as mobile phase 70% methanol (0.005%acetic acid) and 30% acetic acid 0.05% for ascorbic acid determination and a mixture of methanol,tetrahydrofuran, and acetonitrile (60:30:10 v/v/v) for carotenoid analysis in isocratic mode. Themolecular ion was selected for the quantification in selective ion monitoring (SIM) mode. Ascorbicacid was detected with electrospray ionization probe (ESI) in negative mode, while chemical ionizationatmospheric pressure (APCI) in positive mode was used for the target carotenoids. The methodologyfor ascorbic acid analysis is based on an extraction with polytron using methanol and a mixture ofmethaphosphoric acid and acetic acid. Extraction of the carotenoids was carried out with tetrahydrofuran/methanol (1:1) (v/v). The proposed methods were applied, after their correspondingvalidations, to the analysis of four varieties of tomatoes, tomato in tin enriched and dried tomato, andto the analysis of mango and kiwi fruits, to compare the content in these compounds. Moreover, theinfluence of the process of freezing and the effect that the manipulation/preservation has in the contentof ascorbic acid in tomato have also been studied.Keywords: Ascorbic acid; carotenoids; liquid chromatography; mass spectrometry; vegetables; fruits
-carotene, invegetables and fruits by liquid chromatography coupled with mass spectrometry (LC-MS) have beenestablished. The chromatographic separation of the studied compounds and their MS parameterswere optimized to improve selectivity and sensitivity. In both methods, separation was carried outwith two coupled columns, first a C18 and then a dC18, using as mobile phase 70% methanol (0.005%acetic acid) and 30% acetic acid 0.05% for ascorbic acid determination and a mixture of methanol,tetrahydrofuran, and acetonitrile (60:30:10 v/v/v) for carotenoid analysis in isocratic mode. Themolecular ion was selected for the quantification in selective ion monitoring (SIM) mode. Ascorbicacid was detected with electrospray ionization probe (ESI) in negative mode, while chemical ionizationatmospheric pressure (APCI) in positive mode was used for the target carotenoids. The methodologyfor ascorbic acid analysis is based on an extraction with polytron using methanol and a mixture ofmethaphosphoric acid and acetic acid. Extraction of the carotenoids was carried out with tetrahydrofuran/methanol (1:1) (v/v). The proposed methods were applied, after their correspondingvalidations, to the analysis of four varieties of tomatoes, tomato in tin enriched and dried tomato, andto the analysis of mango and kiwi fruits, to compare the content in these compounds. Moreover, theinfluence of the process of freezing and the effect that the manipulation/preservation has in the contentof ascorbic acid in tomato have also been studied.Keywords: Ascorbic acid; carotenoids; liquid chromatography; mass spectrometry; vegetables; fruits