Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry

详细信息    查看全文

• 作者：Maria-Jose BañulsPilar Jiménez-Meneses ; Albert Meyer ; Jean-Jacques Vasseur ; François Morvan ; Jorge EscorihuelaRosa Puchades ; Ángel Maquieira
• 刊名：Bioconjugate Chemistry
• 出版年：2017
• 出版时间：February 15, 2017
• 年：2017
• 卷：28
• 期：2
• 页码：496-506
• 全文大小：457K
• ISSN：1520-4812

文摘

Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol–ene or thiol–yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.