Comparison of “Polarization Inversion with Spin Exchange at Magic Angle” and “Geometric Analysis of Labeled Alanines” Methods for Transmembrane Helix Alignment

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• 作者：Vitaly V. Vostrikov ; Christopher V. Grant ; Anna E. Daily ; Stanley J. Opella ; Roger E. Koeppe
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：September 24, 2008
• 年：2008
• 卷：130
• 期：38
• 页码：12584-12585
• 全文大小：138K
• 年卷期：v.130,no.38(September 24, 2008)
• ISSN：1520-5126

文摘

Using the model α-helical peptide acetyl−GGALW⁵LALALALALALALW¹⁹LAGA−ethanolamide (“GWALP23”), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11°−13° with respect to the bilayer normal.