Anion Complexation and Transport by Isophthalamide and Dipicolinamide Derivatives: DNA Plasmid Transformation in E. coli

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• 作者：Jason L. Atkins ; Mohit B. Patel ; Megan M. Daschbach ; Joseph W. Meisel ; George W. Gokel
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：August 22, 2012
• 年：2012
• 卷：134
• 期：33
• 页码：13546-13549
• 全文大小：262K
• 年卷期：v.134,no.33(August 22, 2012)
• ISSN：1520-5126

文摘

Tris-arenes based on either isophthalic acid or 2,6-dipicolinic acid have been known for more than a decade to bind anions. Recent studies have also demonstrated their ability to transport various ions through membranes. In this report, we demonstrate two important properties of these simple diamides. First, they transport plasmid DNA into Escherichia coli about 2-fold over controls, where the ampicillin resistance gene is expressed in the bacteria. These studies were done with plasmid DNA (2.6 kilobase (kb)) in JM109 E. coli cells. Second, known methods do not typically transport large plasmids (>15 kb). We demonstrate here that transformation of large pVIB plasmids (i.e., >20 kb) were enhanced over water controls by 10-fold. These results are in striking contrast to the normal decrease in transformation with increasing plasmid size.