Porous Silicon/Photosynthetic Reaction Center Hybrid Nanostructure

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• 作者：Kata Hajdu ; Csilla Gergely ; Marta Martin ; Thierry Cloitre ; L谩szl贸 Zim谩nyi ; Katalin Tenger ; Petro Khoroshyy ; Gabriela Palestino ; Vivechana Agarwal ; Kl谩ra Hern谩di ; Zolt谩n N茅meth ; L谩szl贸 Nagy
• 刊名：Langmuir
• 出版年：2012
• 出版时间：August 14, 2012
• 年：2012
• 卷：28
• 期：32
• 页码：11866-11873
• 全文大小：335K
• 年卷期：v.28,no.32(August 14, 2012)
• ISSN：1520-5827

文摘

The purified photosynthetic reaction center protein (RC) from Rhodobacter sphaeroides R-26 purple bacteria was bound to porous silicon microcavities (PSiMc) either through silane-glutaraldehyde (GTA) chemistry or via a noncovalent peptide cross-linker. The characteristic resonance mode in the microcavity reflectivity spectrum red shifted by several nanometers upon RC binding, indicating the protein infiltration into the porous silicon (PSi) photonic structure. Flash photolysis experiments confirmed the photochemical activity of RC after its binding to the solid substrate. The kinetic components of the intraprotein charge recombination were considerably faster (蟿_fast = 14 (卤9) ms, 蟿_slow = 230 (卤28) ms with the RC bound through the GTA cross-linker and only 蟿_fast = 27 (卤3) ms through peptide coating) than in solution (蟿_fast = 120 (卤3) ms, 蟿_slow = 1387 (卤2) ms), indicating the effect of the PSi surface on the light-induced electron transfer in the protein. The PSi/RC complex was found to oxidize the externally added electron donor, mammalian cytochrome c, and the cytochrome oxidation was blocked by the competitive RC inhibitor, terbutryne. This fact indicates that the specific surface binding sites on the PSi-bound RC are still accessible to external cofactors and an electronic interaction with redox components in the aqueous environment is possible. This new type of biophotonic material is considered to be an excellent model for new generation applications at the interface of silicon-based electronics and biological redox systems designed by nature.