Environmental Conditions Modulate the Switch among Different States of the Hydrophobin Vmh2 from Pleurotus ostreatus

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• 作者：Sara Longobardi ; Delia Picone ; Carmine Ercole ; Roberta Spadaccini ; Luca De Stefano ; Ilaria Rea ; Paola Giardina
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：March 12, 2012
• 年：2012
• 卷：13
• 期：3
• 页码：743-750
• 全文大小：384K
• 年卷期：v.13,no.3(March 12, 2012)
• ISSN：1526-4602

文摘

Fungal hydrophobins are amphipathic, highly surface-active, and self-assembling proteins. The class I hydrophobin Vmh2 from the basidiomycete fungus Pleurotus ostreatus seems to be the most hydrophobic hydrophobin characterized so far. Structural and functional properties of the protein as a function of the environmental conditions have been determined. At least three distinct phenomena can occur, being modulated by the environmental conditions: (1) when the pH increases or in the presence of Ca²⁺ ions, an assembled state, 尾-sheet rich, is formed; (2) when the solvent polarity increases, the protein shows an increased tendency to reach hydrophobic/hydrophilic interfaces, with no detectable conformational change; and (3) when a reversible conformational change and reversible aggregation occur at high temperature. Modulation of the Vmh2 conformational/aggregation features by changing the environmental conditions can be very useful in view of the potential protein applications.