Production of Mutagenic Metabolites by Metarhizium anisopliae

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• 作者：Stuart B. Krasnoff ; Christopher H. Sommers ; Yong-Sun Moon ; Bruno G. G. Donzelli ; John D. Vandenberg ; Alice C. L. Churchill ; Donna M. Gibson
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：September 20, 2006
• 年：2006
• 卷：54
• 期：19
• 页码：7083 - 7088
• 全文大小：77K
• 年卷期：v.54,no.19(September 20, 2006)
• ISSN：1520-5118

文摘

NG-391 (1) and NG-393 (2), previously reported from undescribed Fusarium species as nerve-cellgrowth stimulants, were identified from fermentation extracts of the entomopathogenic fungusMetarhizium anisopliae. These compounds are 7-desmethyl analogues of fusarin C and (8Z)-fusarinC, mutagenic toxins from Fusarium species that contaminate corn. A mutant strain of M. anisopliae(KOB1-3) overproduces 1 and 2 by ca. 10-fold relative to the wild-type strain, ARSEF 2575, fromwhich it was derived. Overproduction of these compounds in KOB1-3 imparts a yellow pigmentationto the culture medium of the fungus. These compounds were inactive at 100 mages/entities/mgr.gif">g/disk in antimicrobialdisk diffusion assays. Compound 1 was inactive at 100 ppm in a mosquitocidal assay. However, liketheir fusarin analogues, 1 and 2 exhibited potent S9-dependent mutagenic activity in the Salmonellamutagenicity test. Discovery of these highly mutagenic mycotoxins in M. anisopliae suggests thatscreening for production of NG-391 and NG-393 in strains that are used as biocontrol agents wouldbe a prudent course of action. The impact of these findings on the use of M. anisopliae as a biocontrolagent is currently unknown and requires further investigation.