Effect of Subculture and Elicitation on Instability of Taxol Production in Taxus sp. Suspension Cultures
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- 作者：Beum Jun Kim ; Donna M. Gibson ; and Michael L. Shuler
- 刊名：Biotechnology Progress
- 出版年：2004
- 出版时间：December 2004
- 年：2004
- 卷：20
- 期：6
- 页码：1666 - 1673
- 全文大小：212K
- 年卷期：v.20,no.6(December 2004)
- ISSN：1520-6033
文摘
The production of secondary metabolites through plant cell suspension cultures ischallenging because the level and pattern of production is often unstable andunpredictable. To investigate the factors affecting instability of secondary metaboliteproduction, high Taxol (paclitaxel)-producing Taxus cultures induced by methyljasmonate elicitation and their low Taxol-producing counterparts were compared withrespect to growth and Taxol production kinetics. With Taxus subcultures we observealternating states of high and low productivity. Parental cultures and their subculturesfrom five different cell lines were used to test whether a high-producing culture growsmore slowly or dies more rapidly than a low-producing one. These cell lines were ofthree types: (1) Taxol-producing with and without methyl jasmonate, (2) Taxol-producing only upon elicitation, and (3) nonproducing. High-producing cultures showgrowth inhibition upon subculture, whereas nonproducing elicited cultures show littlegrowth inhibition. Thus, growth inhibition is primarily due to Taxol or taxaneaccumulation and not a direct result of methyl jasmonate treatment. Through mediaexchange between high- and low-producing cultures, it appears that culture components generated by cells alter culture properties. To assess variability as a functionof culture lineage, two groups of replicate cultures were generated either with a mixingof the parental flasks or segregation of parental flasks at each subculture. Althoughparental culture mixing did not reduce flask-to-flask variation, the production levelof Taxol in subcultures resulting from mixing inocula was sustained at a higher levelrelative to segregated subcultures. The results are consistent with the possibility ofcell signaling within the population that can induce Taxol production.