Bacteriorhodopsin Conjugates as Anchors for Supported Membranes

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• 作者：Manoj K. Sharma ; Harsha Jattani ; and M. Lane Gilchrist ; Jr.
• 刊名：Bioconjugate Chemistry
• 出版年：2004
• 出版时间：July 2004
• 年：2004
• 卷：15
• 期：4
• 页码：942 - 947
• 全文大小：211K
• 年卷期：v.15,no.4(July 2004)
• ISSN：1520-4812

文摘

The sophistication of supported lipid bilayer membranes has increased steadily as new applicationsare being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate.Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin-PEG₃₄₀₀-bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymerconjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-basedanchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres.This method could provide a new route for the stability enhancement of supported lipid bilayermembrane assemblies.