Controlled Density Patterning of Tolylterpyridine-Tagged Oligonucleotides

详细信息    查看全文

• 作者：Nunzio Tuccitto ; Nicoletta Giamblanco ; Sumana Ghosh ; Valentina Spampinato ; Pierre Labbe虂 ; Pascal Dumy ; Silvio Quici ; Giovanni Marletta ; Eric Defrancq ; Antonino Licciardello
• 刊名：Langmuir
• 出版年：2011
• 出版时间：July 19, 2011
• 年：2011
• 卷：27
• 期：14
• 页码：8595-8599
• 全文大小：854K
• 年卷期：v.27,no.14(July 19, 2011)
• ISSN：1520-5827

文摘

An efficient surface anchoring strategy of tolylterpyridine-tagged DNA single strands (ssDNA-ttpy) synthesized on gold electrodes is reported. The method is based on exchange reactions between Fe(II)bis-terpyridine complexed SAMs and ssDNA-ttpy, and allows efficient hybrydization of the cDNA strands. Moreover, by using low-current focused ion beam lithography, micropatterned arrays are obtained, homogeneously covered with ssDNA-ttpy. The surface adsorption kinetics of ssDNA-ttpy, as well as its hybridization efficiency, was monitored by in situ quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The effective confinement of the ssDNA-ttpy at the micrometer level has been monitored by time of flight secondary ion mass spectrometry (ToF-SIMS) and ellipsometric surface imaging experiments, providing laterally resolved chemical and topographic mapping.