Composite System of PLCL Scaffold and Heparin-Based Hydrogel for Regeneration of Partial-Thickness Cartilage Defects

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• 作者：Mihye Kim ; Bohee Hong ; Jongman Lee ; Se Eun Kim ; Seong Soo Kang ; Young Ha Kim ; Giyoong Tae
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：August 13, 2012
• 年：2012
• 卷：13
• 期：8
• 页码：2287-2298
• 全文大小：670K
• 年卷期：v.13,no.8(August 13, 2012)
• ISSN：1526-4602

文摘

Delivering isolated chondrocytes with matrix is a promising approach to promote the cartilage repair. The present study attempted to combine the advantages of porous scaffold and hydrogel in delivering chondrocytes to partial-thickness cartilage defects. An electrospun, gelatin-incorporated PLCL scaffold mechanically similar to natural cartilage was fabricated, and chondrocytes were seeded using an injectable heparin-based hydrogel for efficient cell seeding. The scaffold/hydrogel composite showed more enhanced expression of chondrogenic genes and production of GAGs than those prepared without hydrogel. In addition, significant cartilage formation showing good integration with surrounding, similar to natural cartilage, was observed by scaffold/hydrogel composite system in partial-thickness defects of rabbit knees while no regeneration was observed in control defects. Although no exogenous chondrogenic factors were added, it was evident that the scaffold/hydrogel composite system was highly effective and better than the scaffold alone system without hydrogel for cartilage regeneration both in vitro and in vivo.