Synthesis, Characterization, and Preliminary Biological Study of Poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone)
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- 作者：Wei He ; Kenneth E. Gonsalves ; John H. Pickett ; and Craig Halberstadt
- 刊名：Biomacromolecules
- 出版年：2003
- 出版时间：January 2003
- 年：2003
- 卷：4
- 期：1
- 页码：75 - 79
- 全文大小：725K
- 年卷期：v.4,no.1(January 2003)
- ISSN：1526-4602
文摘
Poly(3-(tert-butoxycarbonyl)-N-vinyl-2-pyrrolidone) has been synthesized and characterized by gel permeationchromatography, Fourier transform infrared spectroscopy, NMR spectroscopy, and thermal analysis. Thepolymer is a chemically amplified photoresist. Arrays of lines with 25 
m width and 25 m spacing weresuccessfully patterned with this polymer by photolithography. Rat fibroblast cells were seeded on thesepatterned surfaces as well as the smooth glass surface. Phase contrast microscopy showed that cells on thepatterned surfaces were strongly aligned and elongated along the grooves as compared to randomly spreadingon the smooth surface. Since controlling cell orientation is critical for the development of advanced formsof tissue repair and cell engineering therapies, for example, peripheral nerve repair, production of tendonand ligament substitutes in vitro, and control of microvascular repair, the described polymer may be usefulfor applications in tissue reconstruction.
m width and 25 m spacing weresuccessfully patterned with this polymer by photolithography. Rat fibroblast cells were seeded on thesepatterned surfaces as well as the smooth glass surface. Phase contrast microscopy showed that cells on thepatterned surfaces were strongly aligned and elongated along the grooves as compared to randomly spreadingon the smooth surface. Since controlling cell orientation is critical for the development of advanced formsof tissue repair and cell engineering therapies, for example, peripheral nerve repair, production of tendonand ligament substitutes in vitro, and control of microvascular repair, the described polymer may be usefulfor applications in tissue reconstruction.