New Anthocyanin鈥揌uman Salivary Protein Complexes

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• 作者：Ra煤l Ferrer-Gallego ; Susana Soares ; Nuno Mateus ; Juli谩n Rivas-Gonzalo ; M. Teresa Escribano-Bail贸n ; Victor de Freitas
• 刊名：Langmuir
• 出版年：2015
• 出版时间：August 4, 2015
• 年：2015
• 卷：31
• 期：30
• 页码：8392-8401
• 全文大小：642K
• ISSN：1520-5827

文摘

The interaction between phenolic compounds and salivary proteins is considered the basis of the poorly understood phenomenon of astringency. Furthermore, this interaction is an important factor in relation to their bioavailability. In this work, interactions between anthocyanin and human salivary protein fraction were studied by mass spectrometry (MALDI-TOF-MS and FIA-ESI-MS) and saturation-transfer difference (STD) NMR spectroscopy. Anthocyanins were able to interact with saliva proteins. The dissociation constant (K_D) between malvidin 3-glucoside and salivary proline-rich proteins was 1.92 mM for the hemiketal form (pH 3.4) and 1.83 mM for the flavylium cation (pH 1.0). New soluble complexes between these salivary proteins and malvidin 3-glucoside were identified for the first time.