Fluorescence Spectroscopy of the Retina for the Screening of Bovine Spongiform Encephalopathy

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• 作者：Ujjal Bhattacharjee ; Catherine Graham ; Stefanie Czub ; Sandor Dudas ; Mark A. Rasmussen ; Thomas A. Casey ; Jacob W. Petrich
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：64
• 期：1
• 页码：320-325
• 全文大小：297K
• ISSN：1520-5118

文摘

Transmissible spongiform encephalopathies (TSE) are progressive, neurodegenerative disorders, of which bovine spongiform encephalopathy (BSE) is of special concern because it is infectious and debilitating to humans. The possibility of using fluorescence spectroscopy to screen for BSE in cattle was explored. Fluorescence spectra from the retinas of experimentally infected BSE-positive cattle with clinical disease were compared with those from both sham-inoculated and non-inoculated BSE-negative cattle. The distinct intensity difference of about 4–10-fold between the spectra of the BSE-positive and the BSE-negative (sham-inoculated and non-inoculated) eyes suggests the basis for a means of developing a rapid, noninvasive examination of BSE in particular and TSEs in general.