Cationic Nanoparticles Induce Nanoscale Disruption in Living Cell Plasma Membranes
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- 作者：Jiumei Chen ; Jessica A. Hessler ; Krishna Putchakayala ; Brian K. Panama ; Damian P. Khan ; Seungpyo Hong ; Douglas G. Mullen ; Stassi C. DiMaggio ; Abhigyan Som ; Gregory N. Tew ; Anatoli N. Lopatin ; James R.
- 刊名：Journal of Physical Chemistry B
- 出版年：2009
- 出版时间：August 13, 2009
- 年：2009
- 卷：113
- 期：32
- 页码：11179-11185
- 全文大小：388K
- 年卷期：v.113,no.32(August 13, 2009)
- ISSN：1520-5207
文摘
It has long been recognized that cationic nanoparticles induce cell membrane permeability. Recently, it has been found that cationic nanoparticles induce the formation and/or growth of nanoscale holes in supported lipid bilayers. In this paper, we show that noncytotoxic concentrations of cationic nanoparticles induce 30−2000 pA currents in 293A (human embryonic kidney) and KB (human epidermoid carcinoma) cells, consistent with a nanoscale defect such as a single hole or group of holes in the cell membrane ranging from 1 to 350 nm2 in total area. Other forms of nanoscale defects, including the nanoparticle porating agents adsorbing onto or intercalating into the lipid bilayer, are also consistent; although the size of the defect must increase to account for any reduction in ion conduction, as compared to a water channel. An individual defect forming event takes 1−100 ms, while membrane resealing may occur over tens of seconds. Patch-clamp data provide direct evidence for the formation of nanoscale defects in living cell membranes. The cationic polymer data are compared and contrasted with patch-clamp data obtained for an amphiphilic phenylene ethynylene antimicrobial oligomer (AMO-3), a small molecule that is proposed to make well-defined 3.4 nm holes in lipid bilayers. Here, we observe data that are consistent with AMO-3 making 3 nm holes in living cell membranes.