Oat mi
lling fractions were examined for concentrations of tota
l pheno
lics, toco
ls, and pheno
lic acidsand in vitro antioxidant activity to determine their potentia
l as dietary antioxidants. Methano
licextracts of pear
ling fractions, f
lour and aspirations from f
laking, and trichomes had high,intermediate, and
low antioxidant activities, respective
ly, eva
luated by the
le">-carotene b
leachingmethod. Pear
ling fractions were a
lso highest in tota
l pheno
lics and toco
ls.
p-Hydroxybenzoic acid,vani
llic acid, caffeic acid, vani
llin,
p-coumaric acid, and feru
lic acid were identified and quantifiedby HPLC. Three avenanthramides and an unidentified feru
late derivative were a
lso detected. Tota
lpheno
lic content was significant
ly corre
lated with antioxidant activity, and regression equationsthat predicted antioxidant activity from pheno
lic and toco
l concentrations were ca
lcu
lated.Antioxidant activity, eva
luated by
le">-carotene b
leaching, was corre
lated with measures of oxygenradica
l absorbance capacity and
low-density
lipoprotein oxidation. These data indicate a potentia
lfor oat products, especia
lly those enriched in outer
layers of the
groat, to contribute to dietary intakesof antioxidant phytonutrients.Keywords: Avena sativa; oat; antioxidant activity; pheno
lic acids; toco
ls