Temperature Determination by EPR at 275 GHz and the Detection of Temperature Jumps in Aqueous Samples
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- 作者：Mykhailo Azarkh ; Edgar J. J. Groenen
- 刊名：Journal of Physical Chemistry B
- 出版年：2015
- 出版时间：October 22, 2015
- 年：2015
- 卷：119
- 期：42
- 页码：13416-13421
- 全文大小：286K
- ISSN：1520-5207
文摘
Second-moment analysis along two dimensions of continuous-wave EPR spectra of nitroxides enables EPR thermometry in a broad temperature range. Simulations show that the temperature can be derived in both the slow-motion and the fast-motion regime, which is experimentally verified at 275 GHz for H2O/glycerol (50/50% by volume) and pure water. We demonstrate that this tool allows the calibration of temperature jumps induced by infrared laser irradiation of a submicroliter sample in the single-mode cavity of a 275 GHz spectrometer, which prepares for kinetic studies of processes involving paramagnetic species.