Characterization of Tau in Cerebrospinal Fluid Using Mass Spectrometry

详细信息    查看全文

• 作者：Erik Portelius ; Sara F. Hansson ; Ai Jun Tran ; Henrik Zetterberg ; Pierre Grognet ; Eugeen Vanmechelen ; Kina Hö ; glund ; Gunnar Brinkmalm ; Ann Westman-Brinkmalm ; Eckhard Nordhoff ; Kaj Blennow ; Johan Gob
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：7
• 期：5
• 页码：2114 - 2120
• 全文大小：555K
• 年卷期：v.7,no.5(May 2008)
• ISSN：1535-3907

文摘

The neurodegenerative disorder Alzheimer’s disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.