Comparison of the Emulsion Characteristics of Rhodococcus erythropolis and Ecsherichia coli SOXC-5 Cells Expressing Biodesulfurization Genes

详细信息    查看全文

• 作者：Abhijeet P. Borole ; Eric N. Kaufman ; Matthew J. Grossman ; Vera Minak-Bernero ; Richard Bare ; and M. Kathryn Lee
• 刊名：Biotechnology Progress
• 出版年：2002
• 出版时间：February 2002
• 年：2002
• 卷：18
• 期：1
• 页码：88 - 93
• 全文大小：149K
• 年卷期：v.18,no.1(February 2002)
• ISSN：1520-6033

文摘

Biodesulfurization of fuel oils is a two-phase (oil/water) process which may offer aninteresting alternative to conventional hydrodesulfurization due to the mild operatingconditions and reaction specificity afforded by the biocatalyst. For biodesulfurizationto realize commercial success, a variety of process considerations must be addressedincluding reaction rate, emulsion formation and breakage, biocatalyst recovery, andboth gas and liquid mass transport. This study evaluates emulsion formation andbreakage using two biocatalysts with differing hydrophobic characteristics. A Gram-positive (Rhodococcus erythropolis) biocatalyst, expressing the complete 4S desulfurization pathway, and a Gram-negative biocatalyst (Escherichia coli), expressing onlythe gene for conversion of dibenzothiophene (DBT) to DBT sulfone, are comparedrelative to their ability to convert DBT and the ease of phase separation as well asbiocatalyst recovery following desulfurization.