Non-Equilibrium Isomer Distribution of the Gas-Phase Photoactive Yellow Protein Chromophore
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- 作者：Mitra Almasian ; Josipa Grzetic ; Johanne van Maurik ; Jeffrey D. Steill ; Giel Berden ; Steen Ingemann ; Wybren Jan Buma ; Jos Oomens
- 刊名：The Journal of Physical Chemistry Letters
- 出版年：2012
- 出版时间：August 16, 2012
- 年：2012
- 卷：3
- 期：16
- 页码：2259-2263
- 全文大小：307K
- 年卷期：v.3,no.16(August 16, 2012)
- ISSN：1948-7185
文摘
The conjugate base of para-coumaric acid, which can be conveniently generated in the gas phase by electrospray ionization (ESI), is a commonly used model system for the chromophore of the photoactive yellow protein. Here we report its gas-phase IR spectrum, which shows that the anion easily adopts a carboxylate structure lying 60 kJ/mol higher in energy than the global minimum phenoxide structure. Generation of the biologically more relevant phenoxide isomer by ESI can be achieved using dry acetonitrile as solvent.