Quinone-Modified Mn-Doped ZnS Quantum Dots for Room-Temperature Phosphorescence Sensing of Human Cancer Cells That Overexpress NQO1

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• 作者：Yi-Ming Sung ; Srivardhan Reddy Gayam ; Pei-Ying Hsieh ; Hsin-Yun Hsu ; Eric Wei-Guang Diau ; Shu-Pao Wu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 25, 2015
• 年：2015
• 卷：7
• 期：46
• 页码：25961-25969
• 全文大小：620K
• ISSN：1944-8252

文摘

Early detection of cancer cells in a rapid and sensitive approach is one of the great challenges in modern clinical cancer care. This study has demonstrated the first example of a rapid, selective, and sensitive phosphorescence probe based on phosphorescence energy transfer (PET) for cancer-associated human NAD(P)H:quinone oxidoreductase isozyme 1 (NQO1). An efficient room-temperature phosphorescence NQO1 probe was constructed by using Mn-doped ZnS quantum dots (Mn:ZnS QDs) as donors and trimethylquinone propionic acids as acceptors. Phosphorescence quenching of Mn:ZnS QDs from the Mn:ZnS QDs to a covalently bonded quinone was achieved through PET. Phosphorescence of Mn:ZnS QDs was turned on by the rapid reduction-initiated removal of the quinone quencher by NQO1. This probe shows low cellular toxicity and can rapidly distinguish between NQO1-expressing and -nonexpressing cancer cell lines through phosphorescence imaging.