Dissecting Glycosylation Steps in Lobophorin Biosynthesis Implies an Iterative Glycosyltransferase
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- 作者：Sumei Li ; Ji Xiao ; Yiguang Zhu ; Guangtao Zhang ; Chunfang Yang ; Haibo Zhang ; Liang Ma ; Changsheng Zhang
- 刊名：Organic Letters
- 出版年：2013
- 出版时间：March 15, 2013
- 年：2013
- 卷：15
- 期：6
- 页码：1374-1377
- 全文大小：271K
- 年卷期：v.15,no.6(March 15, 2013)
- ISSN：1523-7052
文摘
The identification of a lobophorin biosynthetic gene cluster from the deep-sea derived Streptomyces sp. SCSIO 01127 reveals a paradigm of three glycosyltransferases (GTs) LobG1鈥揕obG3 being responsible for appending four sugars. Characterization of five differentially glycosylated metabolites from three GT gene-inactivation mutants allowed the assignment of GT functions and the implication of LobG3 as an iterative GT to attach two digitoxoses.