Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization

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• 作者：Jiayun Xiang ; Xiaolei Cai ; Xiaoding Lou ; Guangxue Feng ; Xuehong Min ; Wenwen Luo ; Bairong He ; Chi Ching Goh ; Lai Guan Ng ; Jian Zhou ; Zujin Zhao ; Bin Liu ; Ben Zhong Tang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：7
• 期：27
• 页码：14965-14974
• 全文大小：578K
• ISSN：1944-8252

文摘

Fluorescent organic dots are emerging as promising bioimaging reagents because of their high brightness, good photostability, excellent biocompatibility, and facile surface functionalization. Organic dots with large two-photon absorption (TPA) cross sections are highly desired for two-photon fluorescence microscopy. In this work, we report two biocompatible and photostable organic dots fabricated by encapsulating tetraphenylethene derivatives within DSPE-PEG matrix. The two organic dots show absorption maxima at 425 and 483 nm and emit green and red fluorescence at 560 and 645 nm, with high fluorescence quantum yields of 64% and 22%, respectively. Both organic dots exhibit excellent TPA property in the range of 800鈥?60 nm, affording upon excitation at 820 nm remarkably large TPA cross sections of 1.2 脳 10⁶ and 2.5 脳 10⁶ GM on the basis of dot concentration. The bare fluorophores and their organic dots are biocompatible and have been used to stain living cells for one- and two-photon fluorescence bioimagings. The cRGD-modified organic dots can selectively target integrin 伪_v尾₃ overexpressing breast cancer cells for targeted imaging. The organic dots are also applied for real-time two-photon fluorescence in vivo visualization of the blood vasculature of mouse ear, providing the spatiotemporal information about the whole blood vascular network. These results demonstrate that the present fluorescent organic dots are promising candidates for living cell and tissue imaging.