Glucose Oxidase Immobilization on Guar Gum鈥揋elatin Dual-Templated Silica Hybrid Xerogel

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• 作者：Vandana Singh ; Devendra Singh
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2014
• 出版时间：March 12, 2014
• 年：2014
• 卷：53
• 期：10
• 页码：3854-3860
• 全文大小：340K
• 年卷期：v.53,no.10(March 12, 2014)
• ISSN：1520-5045

文摘

Hybrid xerogel (H5) crafted from guar gum鈥揼elatin dual-templated polymerization of tetramethoxysilane (TMOS) behaved as an effective carrier support for glucose oxidase (GOX). H5 has been characterized using Fourier transform spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM), BET surface area studies, and Zeta potential and particle size distribution analysis. Under optimized conditions, H5鈥揋OX showed a much higher bioactivity (20.73 U mg^鈥?) than free GOX (9.26 U mg^鈥?). The kinetic parameters of free GOX (K_M = 13.988 mg mL^鈥?, V_max = 1.785 渭mole mL^鈥? min^鈥?) and H5鈥揋OX (K_M = 5.403 mg mL^鈥?, V_max = 1.75 渭mole mL^鈥? min^鈥?) revealed that immobilization could enhance the substrate affinity of GOX, although it did not alter the enzymatic reaction rate. H5鈥揋OX retained 96% of its initial bioactivity with 15 days of storage as a dry solid at room temperature. Recycling of H5鈥揋OX showed only marginal loss in bioactivity up to six cycles.