文摘
Hybrid xerogel (H5) crafted from guar gum鈥揼elatin dual-templated polymerization of tetramethoxysilane (TMOS) behaved as an effective carrier support for glucose oxidase (GOX). H5 has been characterized using Fourier transform spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM), BET surface area studies, and Zeta potential and particle size distribution analysis. Under optimized conditions, H5鈥揋OX showed a much higher bioactivity (20.73 U mg鈥?) than free GOX (9.26 U mg鈥?). The kinetic parameters of free GOX (KM = 13.988 mg mL鈥?, Vmax = 1.785 渭mole mL鈥? min鈥?) and H5鈥揋OX (KM = 5.403 mg mL鈥?, Vmax = 1.75 渭mole mL鈥? min鈥?) revealed that immobilization could enhance the substrate affinity of GOX, although it did not alter the enzymatic reaction rate. H5鈥揋OX retained 96% of its initial bioactivity with 15 days of storage as a dry solid at room temperature. Recycling of H5鈥揋OX showed only marginal loss in bioactivity up to six cycles.