Antimicrobial Cellobiose Dehydrogenase-Chitosan Particles

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• 作者：Gregor Tegl ; Barbara Thallinger ; Bianca Beer ; Christoph Sygmund ; Roland Ludwig ; Alexandra Rollett ; Gibson S. Nyanhongo ; Georg M. Guebitz
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：8
• 期：1
• 页码：967-973
• 全文大小：327K
• ISSN：1944-8252

文摘

Increasing prevalence of chronic wounds and microbial infection constitute a severe health challenge. The situation is further complicated by emerging multidrug resistance making the treatment of infections increasingly difficult. Here, a novel antimicrobial system based on in situ release of hydrogen peroxide (H₂O₂) by cellobiose dehydrogenase (CDH) immobilized on chitosan (CTS) particles is described. Covalent immobilization using carbodiimide coupling lead to a higher amount of protein immobilized on CTS (104 μg CDH/mg CTS) when compared to noncovalent immobilization, which, however, showed highest recovery of CDH activity (0.01 U/mg CTS). The CDH-CTS in situ generated H₂O₂ completely inhibited growth of Escherichia coli and Staphylococcus aureus over a period of 24 h. This resilient antimicrobial system represents a novel strategy for preventing infection with potential application in counteracting microbial colonization of chronic wounds.