Structural Characterization and Cytotoxic Properties of an Apiose-Rich Pectic Polysaccharide Obtained from the Cell Wall of the Marine Phanerogam Zostera marina

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• 作者：Vincent Gloaguen ; Vronique Brudieux ; Brigitte Closs ; Aline Barbat ; Pierre Krausz ; Odile Sainte-Catherine ; Michel Kraemer ; Emmanuel Maes ; Yann Guerardel
• 刊名：Journal of Natural Products
• 出版年：2010
• 出版时间：June 25, 2010
• 年：2010
• 卷：73
• 期：6
• 页码：1087-1092
• 全文大小：309K
• 年卷期：v.73,no.6(June 25, 2010)
• ISSN：1520-6025

文摘

Zosterin, an apiose-rich pectic polysaccharide, was extracted and purified from the sea grass Zostera marina. Structural studies conducted by gas chromatography and NMR spectroscopy on a purified zosterin fraction (AGU) revealed a typical apiogalacturonan structure comprising an α-1,4-d-galactopyranosyluronan backbone substituted by 1,2-linked apiofuranose oligosaccharides and single apiose residues. The average molecular mass of AGU was estimated to be about 4100 Da with a low polydispersity. AGU inhibited proliferation of A431 human epidermoid carcinoma cells with an approximate IC₅₀ value of 3 μg/mL (0.7 μM). In addition, AGU inhibited A431 cell migration and invasion. Preliminary experiments showed that inhibition of metalloproteases expression could play a role in these antimigration and anti-invasive properties. Autohydrolysis of AGU, which eliminated apiose and oligo-apiose substituents, led to a virtual disappearance of cytotoxic properties, thus suggesting a direct structure−function relationship with the apiose-rich hairy region of AGU.