Preparation, Characterization, and Time-Resolved Fluorometric Application of Silica-Coated Terbium(III) Fluorescent Nanoparticles

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• 作者：Zhiqiang Ye ; Mingqian Tan ; Guilan Wang ; and Jingli Yuan
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：February 1, 2004
• 年：2004
• 卷：76
• 期：3
• 页码：513 - 518
• 全文大小：129K
• 年卷期：v.76,no.3(February 1, 2004)
• ISSN：1520-6882

文摘

Novel silica-coated terbium(III) chelate fluorescent nanoparticles have been prepared and characterized as a newtype of fluorescence probe for highly sensitive time-resolved fluorescence bioassay. The preparation wascarried out in a water-in-oil microemulsion containing astrongly fluorescent Tb³⁺ chelate, N,N,N¹,N¹-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-phenylpyridine]tetrakis(acetate)-Tb³⁺, Triton X-100, hexanol, and cyclohexane by controlling hydrolysis of tetraethyl orthosilicate. The nanoparticlesare spherical and uniform in size, 42 ± 3 nm in diameter,strongly fluorescent, and highly photostable and haveenough of a long fluorescence lifetime (1.52 ms) for time-resolved fluorescence measurement. A stable and nontoxic method was developed for the surface modificationand protein immobilization of the nanoparticles. As amodel of application, the nanoparticle-labeled streptavidinwas prepared and used in a sandwich-type time-resolvedfluoroimmunoassay of human prostate-specific antigen(PSA) by using a 96-well microtiter plate as the solid-phase carrier. The method gives a detection limit of 7.0pg/mL for the PSA assay.