Preparation of Biotinylated Glyconanoparticles via a Photochemical Process and Study of Their Bioconjugation to Streptavidin

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• 作者：Ravin Narain ; Abdelghani Housni ; Guillaume Gody ; Paul Boullanger ; Marie-Thé ; rè ; se Charreyre ; Thierry Delair
• 刊名：Langmuir
• 出版年：2007
• 出版时间：December 18, 2007
• 年：2007
• 卷：23
• 期：26
• 页码：12835 - 12841
• 全文大小：186K
• 年卷期：v.23,no.26(December 18, 2007)
• ISSN：1520-5827

文摘

We report here the preparation of novel biotinylated glyconanoparticles from well-defined biotinylated glycopolymersand poly(N-isopropylacrylamide) (PNIPAAm) synthesized via the reversible addition fragmentation chain transfer(RAFT) polymerization process. The in situ reduction of the biotinylated glycopolymers, PNIPAAm, poly(ethyleneglycol), and HAuCl₄ via a photochemical process resulted in the formation of biotinylated gold nanoparticles. Themultifunctional biotinylated glyconanoparticles were then evaluated for their bioconjugation toward streptavidin usingUV-vis spectroscopy and surface plasmon resonance (SPR). The biotinylated nanoparticles underwent aggregationin the presence of streptavidin as revealed by spectrophotometry, which indicates the accessibility of the biotin forconjugation. These results were further confirmed by surface plasmon resonance even in the case of surface-immobilizedstreptavidin.