Impact of Polyethylenimine Conjugation Mode on the Cell Transfection Efficiency of Silica Nanovectors

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• 作者：Xiaolin Wang ; Sylvie Masse ; Guillaume Laurent ; Christophe H茅lary ; Thibaud Coradin
• 刊名：Langmuir
• 出版年：2015
• 出版时间：October 13, 2015
• 年：2015
• 卷：31
• 期：40
• 页码：11078-11085
• 全文大小：378K
• ISSN：1520-5827

文摘

The conjugation of polyethylenimine (PEI) to silica nanoparticles has emerged as a useful strategy in gene delivery. Here we investigate the influence of the PEI conjugation mode on the transfection ability of plain silica nanoparticles. Surface functionalization with sulfonate- and chloride-bearing silanes modulates the amount and conformation of PEI and therefore the particles鈥?affinity for the plasmid, without impacting on cytotoxicity. However, transfection efficiency in both immortalized and primary cells is more directly correlated to the nature and strength of the particle鈥揚EI interactions. It suggests that PEI detachment from the particle surface at the stage of endosomal escape is a key event in the plasmid delivery process. These data should provide fruitful guidelines for the fine tuning of colloidal surfaces intended for intracellular delivery of bioactive molecules.