Footprinting of Protein Interactions by Tritium Labeling

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• 作者：Guillaume Mousseau ; Quentin Raffy ; Olivier P. Thomas ; Morgane Agez ; Robert Thai ; Jean Philippe Renault ; Serge Pin ; Franoise Ochsenbein ; Jean-Christophe Cintrat ; Bernard Rousseau
• 刊名：Biochemistry
• 出版年：2010
• 出版时间：May 25, 2010
• 年：2010
• 卷：49
• 期：20
• 页码：4297-4299
• 全文大小：670K
• 年卷期：v.49,no.20(May 25, 2010)
• ISSN：1520-4995

文摘

A new footprinting method for mapping protein interactions has been developed, using tritium as a radioactive label. As residues involved in an interaction are less labeled when the complex is formed, they can be identified via comparison of the tritium incorporation of each residue of the bound protein with that of the unbound one. Application of this footprinting method to the complex formed by the histone H3 fragment H3_122−135 and the protein hAsf1A₁₋₁₅₆ afforded data in good agreement with NMR results.