Physicochemical and Functional Characterization of the Collagen鈥揚olyvinylpyrrolidone Copolymer

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• 作者：Gerardo Leyva-G贸mez ; Enrique Lima ; Guillermo Kr枚tzsch ; Rosario Pacheco-Mar铆n ; Nayeli Rodr铆guez-Fuentes ; David Quintanar-Guerrero ; Edgar Kr枚tzsch
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：August 7, 2014
• 年：2014
• 卷：118
• 期：31
• 页码：9272-9283
• 全文大小：695K
• ISSN：1520-5207

文摘

Collagen鈥損olyvinylpyrrolidone (C鈥揚VP) is a copolymer that is generated from the 纬 irradiation of a mixture of type I collagen and low-molecular-weight PVP. It is characterized by immunomodulatory, fibrolytic, and antifibrotic properties. Here, we used various physicochemical and biological strategies to characterize the structure, biochemical susceptibility, as well as its effects on metabolic activity in fibroblasts. C鈥揚VP contained 16 times more PVP than collagen, but only 55.8% of PVP was bonded. Nevertheless, the remaining PVP exerted strong structural activity due to the existence of weak bonds that provided shielding in the NMR spectra. On SEM and AFM, freeze-dried C鈥揚VP appeared as a film that uniformly covered the collagen fibers. Size analysis revealed the presence of abundant PVP molecules in the solution of the copolymer with a unique dimension related to macromolecular combinations. Calorimetric analysis showed that the copolymer in solution exhibited structural changes at 110 掳C, whereas the lyophilized form showed such changes at temperatures below 50 掳C. The copolymer presented a rheopectic behavior, with a predominant effect of the collagen. C鈥揚VP had biological effects on the expression of integrin 伪₂ and prolyl-hydroxylase but did not interact with cells through the collagen receptors because it did not inhibit or slow contraction.