Immunoassay Reagents for Thyroid Testing. 2. Binding Properties and Energetic Parameters of a T4 Monoclonal Antibody and Its Fab Fragment with a Library of Thyroxine Analog Biosensors Using

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• 作者：Maciej Adamczyk ; John C. Gebler ; Angelo H. Gunasekera ; Phillip G. Mattingly ; and You Pan
• 刊名：Bioconjugate Chemistry
• 出版年：1997
• 出版时间：March 1997
• 年：1997
• 卷：8
• 期：2
• 页码：133 - 145
• 全文大小：354K
• 年卷期：v.8,no.2(March 1997)
• ISSN：1520-4812

文摘

A library of 16 thyroxine analog biosensors were prepared for use on theBIAcore surface plasmonresonance instrument. An anti-thyroxine monoclonal antibody andits Fab fragment were tested witheach biosensor to generate the association and dissociation rateconstants, equilibrium associationconstants, and Gibbs free energy values for characterizing the bindingevent.