Single-Molecule Surface Enhanced Resonance Raman Spectroscopy of the Enhanced Green Fluorescent Protein

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• 作者：Satoshi Habuchi ; Mircea Cotlet ; Roel Gronheid ; Gunter Dirix ; Jan Michiels ; Jos Vanderleyden ; Frans C. De Schryver ; and Johan Hofkens
• 刊名：Journal of the American Chemical Society
• 出版年：2003
• 出版时间：July 16, 2003
• 年：2003
• 卷：125
• 期：28
• 页码：8446 - 8447
• 全文大小：61K
• 年卷期：v.125,no.28(July 16, 2003)
• ISSN：1520-5126

文摘

In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.