Magnetic Bead Sensing Platform for the Detection of Proteins

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• 作者：Randy De Palma ; Gunter Reekmans ; Chengxun Liu ; Roel Wirix-Speetjens ; Wim Laureyn ; Olle Nilsson ; Liesbet Lagae
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：November 15, 2007
• 年：2007
• 卷：79
• 期：22
• 页码：8669 - 8677
• 全文大小：322K
• 年卷期：v.79,no.22(November 15, 2007)
• ISSN：1520-6882

文摘

Over the past 5 years, the on-chip detection and manipulation of magnetic beads via magnetoelectronics hasemerged as a promising new biosensor platform. Magneticbead sensing (MBS) provides a highly sensitive andspecific technique, enabling these sensors to meet thediagnostic needs that are currently not met by existingtechnologies. Although many studies have proven the highphysical sensitivity of magnetic sensors, the establishmentof dose-response curves using MBS is unexplored andtheir capability to sensitively detect low concentrations oftarget molecules for diagnostic applications has remainedunproven. In this study, we have exploited an alternativeMBS concept based on the repositioning of the magneticbeads toward the most sensitive location on the spin valvesensors to allow for highly sensitive immunosensing overa wide range of target concentrations. Furthermore, wepresent the optimization of the magnetoimmuno assay,i.e., the surface chemistry, the blocking procedure, andthe type of magnetic particle, for the highly sensitive andspecific detection of S100, a diagnostic marker forstroke and minor head injury. Finally, a dose-responsecurve was established that illustrates that our MBSplatform can specifically detect S100 down to 27 pg/mL, while maintaining a broad dynamic detection rangeof ~2 decades.