Turn-On Fluorescence Sensor for Intracellular Imaging of Glutathione Using g-C3N4 Nanosheet鈥揗nO2 Sandwich Nanocomposite

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• 作者：Xiao-Long Zhang ; Cheng Zheng ; Shan-Shan Guo ; Juan Li ; Huang-Hao Yang ; Guonan Chen
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：April 1, 2014
• 年：2014
• 卷：86
• 期：7
• 页码：3426-3434
• 全文大小：489K
• 年卷期：v.86,no.7(April 1, 2014)
• ISSN：1520-6882

文摘

Herein, a novel fluorescence sensor based on g-C₃N₄ nanosheet鈥揗nO₂ sandwich nanocomposite has been developed for rapid and selective sensing of glutathione (GSH) in aqueous solutions, as well as living cells. The graphitic-phase C₃N₄ (g-C₃N₄) nanosheet used here is a new type of carbon-based nanomaterial with high fluorescence quantum yield and high specific surface area. We demonstrate a facile one-step approach for the synthesis of a g-C₃N₄ nanosheet鈥揗nO₂ sandwich nanocomposite for the first time. The fluorescence of g-C₃N₄ nanosheet in this nanocomposite is quenched, which attributing to fluorescence resonance energy transfer (FRET) from a g-C₃N₄ nanosheet to the deposited MnO₂. Upon the addition of GSH, MnO₂ is reduced to Mn²⁺, which leads to the elimination of FRET. As a result, the fluorescence of g-C₃N₄ nanosheet is restored. Importantly, the chemical response of the g-C₃N₄鈥揗nO₂ nanocomposite exhibits great selectivity toward GSH relative to other electrolytes and biomolecules. Under the optimal conditions, the detection limit of 0.2 渭M for GSH in aqueous solutions can be reached. Furthermore, the g-C₃N₄鈥揗nO₂ nanocomposite is confirmed to be membrane-permeable and have low cytotoxicity. Moreover, we successfully apply this sensor for visualizing and monitoring change of the intracellular GSH in living cells. Moreover, the proposed sensor shows satisfying performance, such as low cost, easy preparation, rapid detection, good biocompatibility, and turn-on fluorescence response.