Use of Polymorphisms in the 纬-Gliadin Gene of Spelt and Wheat as a Tool for Authenticity Control
详细信息 查看全文
- 作者：Franz Mayer ; Ilka Haase ; Annika Graubner ; Friederike Heising ; Angelika Paschke-Kratzin ; Markus Fischer
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：February 15, 2012
- 年：2012
- 卷：60
- 期：6
- 页码：1350-1357
- 全文大小：368K
- 年卷期：v.60,no.6(February 15, 2012)
- ISSN：1520-5118
文摘
Partial sequencing of the 纬-gliadin gene of 62 spelt and 14 soft wheat cultivars was performed. Fifty-six of the 62 spelt cultivars and 13 of the 14 soft wheat cultivars were shown to exhibit the typical spelt or soft wheat 纬-gliadin sequence, respectively. Exceptions were ascribed to crossbreeding of soft wheat and spelt. Using the typical soft wheat 纬-gliadin sequence, two alternative DNA-based analytical methods were developed for the detection and quantification of spelt flour 鈥渁dulteration鈥?with soft wheat. A simple and fast detection of soft wheat in spelt flours could be achieved by restriction fragment length (RFLP) analysis. In combination with lab-on-a-chip capillary gel electrophoresis (LOC-CE) the soft wheat proportion could be estimated. Heteroduplex formation served as additional confirmation for the presence of spelt besides soft wheat. Hence, RFLP-LOC-CE constitutes a perfect analysis tool for the quality control of cereal seeds and pure cultivars. A precise quantification of soft wheat 鈥渁dulterations鈥?in spelt flour down to 1% could be achieved by the developed real-time PCR method. The calibration parameters of the real-time PCR assay fulfilled the minimum performance requirements of the European Network of GMO (genetically modified organisms) Laboratories (ENGL).