Loop-Mediated Isothermal Amplification (LAMP): Methods for Plant Species Identification in Food
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- 作者：Felix Focke ; Ilka Haase ; Markus Fischer
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：March 27, 2013
- 年：2013
- 卷：61
- 期：12
- 页码：2943-2949
- 全文大小：250K
- 年卷期：v.61,no.12(March 27, 2013)
- ISSN：1520-5118
文摘
Loop-mediated isothermal amplification (LAMP) is a DNA-based analytical method that can be used as an isothermal alternative to polymerase chain reaction (PCR). In comparison to PCR, the advantage of LAMP is the possibility to perform the isothermal reaction without any sophisticated technical equipment; only a water bath is needed, and naked eye detection is sufficient. Up to now, an application of LAMP methods for the detection of even closely related plant species in food or feed matrices has not been described, whereas a large number of PCR methods for that topic are cited in the literature. The aim of the study was the evaluation of LAMP-based methods for plant species identification with respect to method parameters such as R2, LOD, and LOQ. An existing (real-time) PCR method (for the detection of spices) was used for comparison. It could be shown that the developed LAMP methods have potential as alternative strategies to PCR in DNA-based analysis.