Comparative Study of the Folding/Unfolding Dynamics of Poly(glutamic acid) in Light and Heavy Water

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• 作者：Lucille Mendon莽a ; Andreas Steinbacher ; Rapha毛l Bouganne ; Fran莽ois Hache
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：May 22, 2014
• 年：2014
• 卷：118
• 期：20
• 页码：5350-5356
• 全文大小：306K
• 年卷期：v.118,no.20(May 22, 2014)
• ISSN：1520-5207

文摘

The folding/unfolding equilibrium is investigated in poly(glutamic acid) (PGA) by two complementary sets of experiments: temperature-dependent steady-state circular dichroism spectra on the one hand and time-resolved circular dichroism measurements coupled with a T-jump experiment on the other hand. The experiments are performed for PGA dissolved in water for various pH values, as well as in heavy water. The kinetic and thermodynamic parameters extracted from these measurements are shown to be markedly different between light and heavy water, which is assigned to the difference in hydrogen bond energies in both solvents.