Portable, Constriction–Expansion Blood Plasma Separation and Polymerization-Based Malaria Detection

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• 作者：Tatyana A. Shatova ; Shefali Lathwal ; Marissa R. Engle ; Hadley D. Sikes ; Klavs F. Jensen
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：August 2, 2016
• 年：2016
• 卷：88
• 期：15
• 页码：7627-7632
• 全文大小：311K
• 年卷期：0
• ISSN：1520-6882

文摘

A portable, microfluidic blood plasma separation device is presented featuring a constriction–expansion design, which produces 100.0% purity for undiluted blood at 9% yield. This level of purity represents an improvement of at least 1 order of magnitude with increased yield compared to that achieved previously using passive separation. The system features high flow rates, 5–30 μL/min plasma collection, with minimal clogging and biofouling. The simple, portable blood plasma separation design is hand-driven and can easily be incorporated with microfluidic or laboratory scale diagnostic assays. The separation system was applied to a paper-based diagnostic test for malaria that produced an amplified color change in the presence of Plasmodium falciparum histidine-rich protein 2 at a concentration well below clinical relevancy for undiluted whole blood.