Toward a Mechanism for Biliprotein Lyases: Revisiting Nucleophilic Addition to Phycocyanobilin

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• 作者：Jun-Ming Tu ; Ming Zhou ; Rainer Haessner ; Matthias Plscher ; Lutz Eichacker ; Hugo Scheer ; Kai-Hong Zhao
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：April 22, 2009
• 年：2009
• 卷：131
• 期：15
• 页码：5399-5401
• 全文大小：162K
• 年卷期：v.131,no.15(April 22, 2009)
• ISSN：1520-5126

文摘

Biliprotein lyases attach linear-tetrapyrrolic bilins covalently to apoproteins, which is a prerequisite for the assembly of phycobiliproteins into phycobilisomes, the light-harvesting complexes of cyanobacteria. On the basis of the addition of thiol and imidazole to phycocyanobilin, we propose a generalized lyase reaction mechanism. The adducts contain isomerized phycocyanobilin that can be transferred by the lyase to apoproteins by either back-isomerization, generating phycocyanobilin-containing proteins, or direct transfer, generating phycoviolobilin-containing proteins.