Isolation and Characterization of Five Glycerol Esters from Wuhan Propolis and Their Potential Anti-Inflammatory Properties
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- 作者：Haiming Shi ; Haisha Yang ; Xiaowei Zhang ; Yi Sheng ; Haiqiu Huang ; Liangli Yu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：October 10, 2012
- 年：2012
- 卷：60
- 期：40
- 页码：10041-10047
- 全文大小：289K
- 年卷期：v.60,no.40(October 10, 2012)
- ISSN：1520-5118
文摘
Five new glycerol esters including 2-acetyl-1-coumaroyl-3-cinnamoylglycerol (13), (+)-2-acetyl-1-feruloyl-3-cinnamoylglycerol (14), (鈭?-2-acetyl-1-feruloyl-3-cinnamoylglycerol (15), 2-acetyl-1,3-dicinnamoylglycerol (16), and (鈭?-2-acetyl-1-(E)-feruloyl-3-(3鈥?味),16鈥?-dihydroxy-palmitoylglycerol (17) were isolated from methanolic extract of Wuhan propolis. The chemical structures of the five new compounds were confirmed by 1H and 13C NMR and HR-MS spectra. Twelve minor constituents in the Wuhan propolis extract were tentatively identified by UPLC鈥換-TOF-MS, according to their characteristic UV spectrum, retention times, and accurate MS data. The anti-inflammatory activities of the five new compounds were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophage cells. All five compounds exhibited remarkable inhibitory effects on interleukin (IL)-1尾, IL-6, and cyclooxygenase (COX)-2 mRNA expressions at 10 and 100 渭M, suggesting that Wuhan propolis may serve as a potential anti-inflammatory functional food ingredient or nutraceutical.