Photocleavable DNA Barcode鈥揂ntibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

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• 作者：Sarit S. Agasti ; Monty Liong ; Vanessa M. Peterson ; Hakho Lee ; Ralph Weissleder
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：November 14, 2012
• 年：2012
• 卷：134
• 期：45
• 页码：18499-18502
• 全文大小：273K
• 年卷期：v.134,no.45(November 14, 2012)
• ISSN：1520-5126

文摘

DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode鈥揳ntibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout. As a proof of principle, we demonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.