Patterning of Antibodies Using Flexographic Printing

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• 作者：Christopher O. Phillips ; Sridhar Govindarajan ; Simon M. Hamblyn ; R. Steven Conlan ; David T. Gethin ; Tim C. Claypole
• 刊名：Langmuir
• 出版年：2012
• 出版时间：June 26, 2012
• 年：2012
• 卷：28
• 期：25
• 页码：9878-9884
• 全文大小：316K
• 年卷期：v.28,no.25(June 26, 2012)
• ISSN：1520-5827

文摘

Antibodies were patterned onto flexible plastic films using the flexographic printing process. An ink formulation was developed using high molecular weight polyvinyl alcohol in carbonate鈥揵icarbonate buffer. In order to aid both antibody adhesion and the quality of definition in the printed features, a nitrocellulose coating was developed that was capable of being discretely patterned, thus increasing the signal-to-noise ratio of an antibody array. Printing antibody features such as dots, squares, text, and fine lines were reproduced effectively. Furthermore, this process could be easily adapted for printing of other biological materials, including, but not limited to, enzymes, DNA, proteins, aptamers, and cells.