Capillary Electrophoresis of Supercoiled and Linear DNA in Dilute Hydroxyethyl Cellulose Solution

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• 作者：Richard W. Hammond ; Hidehiro Oana ; Jeffrey J. Schwinefus ; Jeffrey Bonadio ; Robert J. Levy ; and Michael D. Morris
• 刊名：Analytical Chemistry
• 出版年：1997
• 出版时间：March 15, 1997
• 年：1997
• 卷：69
• 期：6
• 页码：1192 - 1196
• 全文大小：285K
• 年卷期：v.69,no.6(March 15, 1997)
• ISSN：1520-6882

文摘

Capillary electrophoresis in dilute hydroxyethyl celluloseis shown to separate supercoiled DNA in the size range2000-16 000 base pairs. The plasmids migrate moreslowly than linear ds-DNA of the same sizes. Plasmidbandwidths are larger than observed for ds-DNA, allowingidentification of the type of DNA by bandwidth. Thediffering dependence of mobility on chain length can beexplained by assuming that a plasmid migrates as anelastic rod, while ds-DNA migrates as a wormlike chain.