CMC-Modified Cellulose Biointerface for Antibody Conjugation

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• 作者：Hannes Orelma ; Tuija Teerinen ; Leena-Sisko Johansson ; Susanna Holappa ; Janne Laine
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：April 9, 2012
• 年：2012
• 卷：13
• 期：4
• 页码：1051-1058
• 全文大小：484K
• 年卷期：v.13,no.4(April 9, 2012)
• ISSN：1526-4602

文摘

In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir鈥揝chaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.