Tailored Glycoproteomics and Glycan Site Mapping Using Saccharide-Selective Bioorthogonal Probes

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• 作者：Sarah R. Hanson ; Tsui-Ling Hsu ; Eranthie Weerapana ; Kuniyuki Kishikawa ; Gabriel M. Simon ; Benjamin F. Cravatt ; Chi-Huey Wong
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：June 13, 2007
• 年：2007
• 卷：129
• 期：23
• 页码：7266 - 7267
• 全文大小：74K
• 年卷期：v.129,no.23(June 13, 2007)
• ISSN：1520-5126

文摘

Protein glycosylation is an important post-translational modification of proteins that has profound effects on structure and function. However, the complex and non-templated nature of glycans has been a barrier to studying many of their basic features, especially on a proteome-wide scale. Here, we introduce a glycoproteomic method, glycoprotein identification and glycan mapping (GIDmap), that tailors the isolation of specific glycoprotein subpopulations based on display of metabolically inserted alkynyl sugar probes that can be selectively manipulated using the bioorthogonal Cu(I)-catalyzed [3 + 2] azide-alkyne cycloaddition. This saccharide-selective glycoprotein immobilization allows for subsequent manipulation and analysis of peptides and glycopeptides by liquid chromatography-tandem mass spectrometry. The power of GIDmap was demonstrated by mapping over 200 N-linked glycosylation sites from glycoproteins isolated from prostate cancer cells treated with an alkynyl sugar derivative of N-acetylmannosamine. Overall, GIDmap is a robust method that will greatly aid in inventorying glycoproteins and mapping glycosylation sites, in addition to providing specific information about saccharide content and glycan behavior.